Comparing Effects of BK Virus Agnoprotein and Herpes Simplex-1 ICP47 on MHC-I and MHC-II Expression
Figure 5
(a) IFNγ production by CMVpp65-specific T cells: IFNγ SFUs are shown for T cells from healthy donor 1 (HD1) and 2 (HD2) challenged with medium (neg), phytohemagglutinin (PHA), CMV-pp65-15 m123, and CMV-pp65-9 m495, CMV-pp65-15 Mp. (b) Effect of Tet-off inducible BKV agnoprotein expression on CTL activity. UTA-6 clone 2C9 cells were cultured for 24 h in the presence of Tet (continuous line) or absence of Tet expressing agnoprotein (dotted line, agno), labeled with 51Cr and then pulsed without or with CMV 9-mer peptide NLVPMVATV. CMV antigen-specific T cells from a healthy donor was added in the indicated effector : target ratios and percent release of 51Cr measured.
