Membrane-proximal external region of gp41.
2EBV transformation of B cells.
3V1V2 site on gp120.
4Glycan V3 site on gp120.
5CD4 binding site on GP120.
6Neutralization assays of B cells from infected donors.
7Resurfaced stabilized core 3 probe.
8Somatic mutation primers, gp120 and gp140 probes.
9454 pyrosequencing to characterize additional VRC01-like antibodies from HIV-1—infected individuals.
10Efficient B cell immortalization and high throughput screening.