Allergen-Specific IgG Antibodies Purified from Mite-Allergic Patients Sera Block the IgE Recognition of Dermatophagoides pteronyssinus Antigens: An In Vitro Study
Figure 4
Levels of IgG1 (a), IgG4 (b), and IgE (c) antibodies to Dermatophagoides pteronyssinus (Dpt) allergen extract in the specific (Sp) and nonspecific (Ns) purified IgG fractions obtained from atopic and nonatopic patients, respectively, determined by ELISA. Purified IgG fractions were titrated at two-fold dilutions from 80 to 2.5 µg/well, and data are expressed in ELISA index (EI). The dashed lines indicate the cutoff of the reaction (). The values indicating the Sp/Ns ratio for each antibody class and analyzed concentration are also indicated.
