Impaired Lymphocyte Profile in Schistosomiasis Patients with Periportal Fibrosis
Figure 3
Frequency of T cell populations expressing CD25 molecule. Strategy to analyze the intensity of expression of CD25 molecule on cells. Plot (a) and histogram (b) are representative of one experiment. Frequency of (c), (d), and cells (e) in individuals infected with S. mansoni with different degrees of periportal fibrosis. Ex vivo PBMC were stained for surface markers. The selection of T lymphocyte subsets was initially performed based on their forward (FSC) and side (SSC) light scatter properties, followed by immunophenotyping using a double-labeling platform with anti-CD4-APC and anti-CD25-FITC mAbs and flow cytometry. Results are expressed in median, minimum, maximum, and interquartile values of frequency (*; Kruskal-Wallis test).
