Stimulation of TLR4 by LMW-HA Induces Metastasis in Human Papillary Thyroid Carcinoma through CXCR7
Figure 3
LMW-HA upregulates CXCR7 to promote W3 cell proliferation and migration. ((a)-(b)) W3 cells were incubated with LMW-HA (100 μg/mL) for 24 h; representative flow cytometric analysis of CXCR7 (a) or CXCR4 (b) expression was shown. (c) Scram-W3 cells and TLR4-KD W3 cells were treated with or without LMW-HA (100 μg/mL) for 24 h; representative flow cytometric analysis of CXCR7 expression was shown. (d) W3 cells were transfected with scrambled shRNA (Scram) or CXCR7 shRNA-expressing constructs (CXCR7-KD), and subjected to immunoblot analysis. (e) Scram-W3 cells and CXCR7-KD W3 cells were seeded into 96 well plates (2,000 cells/well) and treated with or without LMW-HA. Cell proliferation was analyzed. Data are mean ± SEM for three independent experiments. (f) Scram-W3 cell and CXCR7-KD W3 cell migration to CXCL12 treated with or without LMW-HA was determined. Data are mean ± SEM for three independent experiments.
