Stimulation of TLR4 by LMW-HA Induces Metastasis in Human Papillary Thyroid Carcinoma through CXCR7
Figure 4
LMW-HA enhances the tumorigenicity of W3 cell line via TLR4/CXCR7 pathway. (a) W3 cells (6 × 106 cell/mouse) were injected subcutaneously into the flanks of nude mice, and mice were intratumorally injected with LMW-HA (400 μg/kg) or the same volume of DMSO every other day. Tumors were measured with a caliper every fourth day. When tumor maximum diameter reached about 1.0 cm, mice were euthanized and tumors were removed and weighed. The volumes of the tumor masses formed in LMW-HA treatment groups and control treatment groups were determined. (b) Scram-W3 cells, TLR4-KD W3 cells, and CXCR7-KD W3 cells were injected subcutaneously into the flanks of nude mice, and mice were intratumorally injected with LMW-HA (400 μg/kg) or the same volume of DMSO every other day. Tumor volumes were determined and shown. (c) CXCR7 expression of tumors was analyzed by immunohistochemistry (original magnification 400×). These results were representative of three independent experiments.
