A Simple Methodology for Conversion of Mouse Monoclonal Antibody to Human-Mouse Chimeric Form

Figure 2

Western blot analysis for the detection of SARS-CoV spike (S) protein (210 kDa). HEK 293-FT cells were transfected with no plasmid (mock) or with plasmid expressing full-length S, pXJ3′-S. Cell lysates were separated in a 7.5% SDS-PAGE gel and detected using mouse mAb 1A9 and human-mouse chimeric mAb 1A9 via Western blot analysis as previously described [23]. Molecular weight markers (in kilodaltons) are indicated on the left.