Protective Effects of HBSP on Ischemia Reperfusion and Cyclosporine A Induced Renal Injury
Figure 7
The expression of caspase-3 protein was measured by western blot (a). There was much more 32 kD precursor transformed to active subunits in the IR + CsA kidneys than that in the normal, ischemia only, or IR kidneys (b) but this was reversed by HBSP treatment. The expression of 17 kD active caspase-3 was upregulated by IR and furthered by CsA decreased by HBSP (c). Another active subunit of 12 kD caspase-3 was predominantly expressed in the ischemia only kidneys, which was decreased after reperfusion in the IR kidneys (d). Data are expressed as the volume density corrected against the loading control of 42 kD β-actin (mean ± SEM; ). ; .
