LMW Heparin Prevents Increased Kidney Expression of Proinflammatory Mediators in (NZBxNZW)F1 Mice

Table 2

Transcriptional levels of cytokines and Toll-like receptors in stimulated splenocytes.

Relative gene expression levels^a in splenocytes from nephritic mice
Genes	Nucleosomes			Nucleosome lmw heparin
	20 hours	4 days	7 days	20 hours	4 days	7 days
IL1β	15.87±5.09	𝟕𝟕.𝟕𝟖±𝟕.𝟗𝟐^∗	𝟕𝟐.𝟔𝟗±𝟐𝟗.𝟎𝟖^∗	15.54±4.62	𝟖𝟔.𝟖𝟒±𝟏𝟑.𝟎𝟗^∗	𝟖𝟎.𝟖𝟔±𝟏𝟎.𝟖𝟎^∗
IL6	1.80±0.42	6.91±2.46	𝟑𝟓.𝟐𝟓±𝟖.𝟎𝟖^∗	1.52±0.57	6.57±1.21	𝟑𝟕.𝟐𝟓±𝟏𝟓.𝟎𝟗^∗
IL10	1.29±0.53	1.90±0.25	𝟑.𝟓𝟓±𝟎.𝟏𝟐^∗	1.47±0.48	1.79±0.21	𝟒.𝟒𝟓±𝟐.𝟏𝟔^∗
IFN-γ	2,35±0.79	4,10±2.62	10,99 ± 0.38*	3,00±1.18	6,53±4.57	11,20 ± 4.03*
TNFα	𝟒.𝟕𝟓±𝟎.𝟑𝟐^∗	𝟓.𝟒𝟓±𝟎.𝟖𝟐^∗	𝟏.𝟗𝟕±𝟎.𝟑𝟒^∗	𝟓.𝟒𝟒±𝟏.𝟐𝟑^∗	𝟖.𝟑𝟕±𝟏.𝟐𝟒^∗	2.04 ± 0.14*
CXCL1	𝟏𝟏𝟎.𝟕𝟎±𝟒𝟕.𝟏𝟔^∗	51.80±11.37	𝟖𝟏.𝟔𝟑±𝟒𝟔.𝟑𝟏^∗	𝟗𝟏.𝟕𝟓±𝟐𝟕.𝟐𝟒^∗	45.92±4.40	𝟗𝟏.𝟕𝟓±𝟐𝟑.𝟑𝟖^∗
TLR2	1.81±0.75^b	𝟏𝟎.𝟗𝟒±𝟎.𝟔𝟓^∗	𝟏𝟗.𝟕𝟎±𝟒.𝟒𝟎^∗	1.69±0.48	𝟏𝟎.𝟏𝟓±𝟏.𝟑𝟗^∗	𝟏𝟗.𝟎𝟐±𝟐.𝟎𝟖^∗
TLR7	𝟐.𝟎𝟗±𝟎.𝟖𝟐^∗	𝟑.𝟐𝟏±𝟎.𝟓𝟕^∗	𝟐.𝟑𝟎±𝟎.𝟑𝟖^∗	1.47±0.41	𝟐.𝟎𝟒±𝟎.𝟏𝟐^∗	𝟐.𝟐𝟑±𝟏.𝟏𝟎^∗
TLR8	𝟎.𝟑𝟒±𝟎.𝟏𝟐^∗	𝟏.𝟔𝟒±𝟎.𝟏𝟗^∗	𝟑.𝟑𝟏±𝟎.𝟎𝟗^∗	0.43±0.23	𝟏.𝟕𝟕±𝟎.𝟑𝟏^∗	𝟐.𝟔𝟏±𝟎.𝟐𝟖^∗
TLR9	1.11±0.23	2.01±0.05	𝟒.𝟎𝟖±𝟎.𝟖𝟕^∗	1.98±1.05	𝟐.𝟕𝟔±𝟎.𝟑𝟔^∗	𝟓.𝟏𝟎±𝟏.𝟕𝟗^∗

aData is given as fold change compared to medium stimulated splenocytes at the same time point. *Statistically significant (𝑃<0.05) change in mRNA levels compared to medium stimulated cells. ^bMean values and SD were calculated from triplicates.