What Are the Molecules Involved in Regulatory T-Cells Induction by Dendritic Cells in Cancer?

Figure 1

Patients’ immature Mo-DCs express higher levels of PD-L1. Blood monocytes from control, and breast cancer patient subjects were cultured in the presence of IL-4 and GM-CSF for seven days and, subsequently, characterized. Flow cytometry analysis showing grouped frequency of CD14^Low/HLADR⁺ (a) and CD11c⁺ (b) cells and Mean Intensity Fluorescence (MFI) values of CD14^Low/HLADR⁺ gated cells to CD80 (c), CD86 (d), and PD-L1 (e) molecules in Mo-iDCs from healthy donors and breast cancer patients (*𝑃<0.05, two-tailed unpaired t-test; healthy 𝑛=5; patients 𝑛=9). (Mature Mo-DCs were activated by TNF-alfa for 48 hours.)

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