Synergy between Vitamin D3 and Toll-Like Receptor Agonists Regulates Human Dendritic Cell Response during Maturation
Figure 1
Vitamin D3 inhibits LPS-induced DC maturation. (a) DC were differentiated in the presence or absence of vitamin D3 and matured with LPS for 4 hrs (upper panel) or 24 hrs (lower panel) prior to flow cytometric analysis. DC were treated without vitamin D3 (vit D) and LPS (red curve), without vitamin D3 and with LPS (blue curve), with vitamin D3 and without LPS (green curve), and with both vitamin D3 and LPS (yellow curve) and were stained with fluorescence-conjugated antibodies HLA-DR(FITC), CD80(PE), CD83(PC5), CD14(FITC), CD40(PE), and CD86(PC5). Histograms represent collection of 5,000 events. (b) Morphology of the DC was examined by light microscopy at ×10 magnification at 4 and 24 hrs after mock or LPS treatment. Experiments are representatives of at least two independent experiments.
