Synergy between Vitamin D3 and Toll-Like Receptor Agonists Regulates Human Dendritic Cell Response during Maturation
Figure 4
LPS and PGN act in synergy with vitamin D3 to enhance expression of the vitamin D receptor. (a) The levels of VDR protein in DC were measured by the western blotting of whole-cell lysates from DC cultures stimulated with either mock, 10 ng/mL LPS, or 10 μg/mL PGN in the acellsbsence or presence of 100 nM vitamin D3. GAPDH was used as a loading control. (b) The band intensity in (a) was quantified and normalized to GAPDH as a VDR/GAPDH ratio (VDR expression).