Potential Immune Modularly Role of Glycine in Oral Gingival Inflammation
Figure 4
Effects of IL-1β and/or glycine on NFκB signaling. (a) Immune fluorescence staining of NFκB signaling protein p65 of cells (A), glycine (B), IL-1β (C), and IL-1β + glycine (D) treated human gingival keratinocytes. The photographs demonstrate a localization of p65 protein primarily in the cell cytoplasm in untreated cells. Untreated controls (A) and glycine treated cells (B) did not affect p65 expression. Following the induction of inflammation by IL-1β administration, NFκB pathway was activated as demonstrated by translocation of p65 protein to the cell nucleus (C). The addition of glycine to the inflammatory cell culture medium reduced the observed p65 protein translocation (D). (b) Semiquantitative assessment of p65 protein translocation in human gingival keratinocytes following administration of IL-1β in combination with and without glycine. difference between treated and control groups following ANOVA analysis; difference between IL-1β and IL-1β + glycine treated groups.
