Immunological Demyelination Triggers Macrophage/Microglial Cells Activation without Inducing Astrogliosis
Figure 3
Macrophage-activation antigens (ED-1) 7 days following intraspinal injection of GalC antibodies plus complement proteins or a spinal cord stab injury to the dorsal column. (a) Eriochrome cyanine R-stained transverse section showing the region of immunological demyelination (*). (b) Double stained GFAP (green) and ED-1 (red) serial section to (a). The inset highlights the numerous ED-1 positive cells within the region of immunological demyelination (white*) and the absence of astrogliosis. (c) Localization of ED-1 positive cells (arrows) within the region of immunological demyelination (hatched line). Activated macrophages characterized by their large round cell bodies and their high immunoreactivity for ED-1 are distributed homogeneously throughout the whole region of immunological demyelination. (d) Eriochrome cyanine R-stained transverse section showing the injury site (I). (e) Double stained GFAP (green) and ED-1 (red) serial section to (d). Numerous ED-1 positive cells are present within the injury site bordered by numerous GFAP positive-hypertrophic astrocytes. (f) Quantitative comparisons between the number of ED-1+ cells per mm2 found within injury epicenters and within regions of immunological demyelination (*), . Scale bars: (a), (d), and (e) 200 μm; (b) and (c) 50 μm.
