A New ELISA for Dermatomyositis Autoantibodies: Rapid Introduction of Autoantigen cDNA to Recombinant Assays for Autoantibody Measurement

<table>ELISA using biotinylated recombinant proteins of 5 different DM-specific autoantigens. Serial dilution of biotinylated <i>in vitro</i> translation and transcription product for ELISA using 2 representative positive sera from DM patients. Serum dilution was 1 : 1,000. Red circles: Mi-2<i>β</i>. Yellow circles: MJ (NXP-2). Purple circles: TIF1-<i>α</i>. Pink circles: MDA5. Green circles: TIF1-<i>γ</i>. RLU = relative luminescence unit.</table>

Journal of Immunology Research

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Figure 3

Figure 3: A New ELISA for Dermatomyositis Autoantibodies: Rapid Introduction of Autoantigen cDNA to Recombinant Assays for Autoantibody Measurement 