932318.fig.005a
(a)
932318.fig.005b
(b)
Figure 5: Detection of residual beads in amplified T cells. (a) Limit of detection of magnetic beads among a T cell population. Variable numbers of beads were mixed with 106 T cells in 1 mL. Beads were detected by their autofluorescence in the various channels of a Facs Canto II. (b) Absence of residual beads in amplified T cells. After 8 days of culture of sorted T cells, each T cell suspension is placed inside a magnet to remove magnetic beads. After two rounds of elimination, the absence of residual beads is checked by flow cytometry.