A Full GMP Process to Select and Amplify Epitope-Specific T Lymphocytes for Adoptive Immunotherapy of Metastatic Melanoma

Table 1

Amplification rates of antigen-specific T cells on irradiated allogeneic feeder cells.

Peptide	Number of; sorted specific cells	Final number of; amplified specific cells	Amplification factor^1	Average number of; cell divisions
Melan-AA27L	10^5	1.16×10^9	1160	10
	8×10^4	2.46×10^8	3075	11
	5×10^3	4.3×10^6	860	10
	5×10^3	1.3×10^7	2600	11
	10^5	1.38×10^9	1380	10
			𝟏𝟖𝟏𝟓±𝟒𝟑𝟐	𝟏𝟎.𝟒±𝟎.𝟓
MELOE-136-44	3×10^5	6×10^8	2000	11
	10^5	1.97×10^8	1970	11
	8×10^4	2.6×10^6	325	8
	2.4×10^4	1.35×10^8	5625	12
	2×10^4	1.8×10^7	900	10
			𝟐𝟏𝟔𝟒±𝟗𝟐𝟑	𝟏𝟎.𝟒±𝟏.𝟓

1Amplification factors are estimated from the final number of amplified T cells divided by the number of rosetted T cells seeded on feeder cells.