The Association of CD81 Polymorphisms with Alloimmunization in Sickle Cell Disease

Table 4

SNP genotype association with alloimmunization in SCD.

SNP	Gene	Genotypes	Number of patients*		𝑃 value	HapMap^#
			Antibody positive	Antibody negative
(1) rs1049388	ARHG	CC/CG/GG	21/8/0	21/11/0	0.593273	62/30/8
(2) rs1451724	ARHG	AA/AG/GG	1/3/27	0/3/29	0.833054	0/23/77
(3) rs4910852	ARHG	AA/AG/GG	25/7/0	18/10/0	0.264449	61/30/9
(4) rs7128013	ARHG	AA/AC/CC	13/12/5	11/18/4	0.543399	25/49/26
(5) rs7929197	ARHG	CC/CT/TT	7/13/12	8/12/5	0.429608	30/45/25
(6) rs10742177	ARHG	CC/CG/GG	10/14/7	18/3/5	0.008979	41/45/14
(7) rs10835182	ARHG	AA/AT/TT	0/14/15	0/12/20	0.444898	2/23/75
(8) rs10835184	ARHG	AA/AT/TT	25/2/0	26/2/2	0.550071	95/5/0
(9) rs12272393	ARHG	AA/AC/CC	7/13/14	8/12/11	0.817062	30/45/25
(10) rs17173879	ARHG	AA/AG/GG	30/2/0	25/1/0	0.999999	93/7/0
(11) rs2231529	CHRNA	CC/CT	27/3/1	35/2/0	0.493847	93/7/0
(12) rs2231532	CHRNA	AA/AG/GG	19/13/3	14/12/5	0.609984	63/35/2
(13) rs2271583	CHRNA	AA/AG/GG	4/14/15	2/9/18	0.422893	10/33/57
(14) rs2672213	CHRNA	CC/CT/TT	30/2/0	23/4/0	0.397881	97/3/0
(15) rs2672216	CHRNA	CC/CT/TT	32/2/0	25/8/1	0.062890	85/13/2
(16) rs2741862	CHRNA	CC/CT/TT	5/25/4	14/14/5	0.022396	23/35/42
(17) rs12221525	CHRNA	CC/CG/GG	20/8/4	20/11/2	0.562563	50/37/13
(18) rs708564 **	CD81	CC/CT/TT	5/15/11	23/8/3	0.000086 ^##	82/17/2
(19) rs731909	CD81	CC/CG/GG	27/6/3	33/2/0	0.064002	90/8/2
(20) rs756915	CD81	CC/CT/TT	9/19/8	12/14/10	0.495260	21/50/29
(21) rs800137	CD81	CC/CT/TT	6/16/13	14/14/7	0.086688	46/31/24
(22) rs800335	CD81	CC/CT/TT	7/10/18	4/8/24	0.374866	82/17/2
(23) rs874330	CD81	CC/CT/TT	13/15/7	19/9/7	0.284287	52/40/8
(24) rs2019938	CD81	AA/AG/GG	11/13/5	15/14/8	0.851861	43/33/23
(25) rs2237863	CD81	CC/CT/TT	3/22/5	15/10/6	0.001518 ^##	15/47/38
(26) rs11022565	CD81	GG/GT/TT	17/14/4	21/7/5	0.280369	61/32/7
(27) rs11022567	CD81	AA/AG/GG	5/14/16	5/7/25	0.120284	7/33/60

∗Patient numbers are displayed in the order shown in the column labeled genotypes. #Relative distribution of genotypes in Sub-Saharan population, as reported by the HapMap. This research utilizes the NCBI SNP database [41], (http://www.ncbi.nih.gov/snp/). The Single Nucleotide Polymorphism database (dbSNP) is a public domain archive for a broad collection of simple genetic polymorphisms. dbSNP reports many cases of SNPs genotyped by HapMap and other projects which provide additional genotype and allele frequency information. ∗∗NCBI database describes two additional alleles, A/G with very low frequencies (<0.5%) for rs708564. This might be due to a mutational mechanism that leads to the simultaneous creation of two new base pairs at the same site which is beyond the scope of this study [42]. Our results, however, are based on the two reference SNPs alleles (C/T) of rs708564. ^##𝑃 values significant comparing SCD patients with and without alloimmunization.