An Elucidation of Neutrophil Functions against Mycobacterium tuberculosis Infection
Figure 1
Intracellular survival of H37Rv in neutrophils treated with NAC and L-GSH ( ). Neutrophils were infected with processed H37Rv at a low dose multiplicity of infection of 1 : 10 (1 bacterium for every 10 neutrophils) and incubated for 2 hours to allow for phagocytosis. Unphagocytosed mycobacteria were removed by washing the infected neutrophils three times with warm sterile PBS. Infected neutrophils were cultured in RPMI + 5% AB serum at 37°C + 5% CO2 in the presence and absence of NAC (10 mM) or L-GSH (10 and 20 μM). Infected neutrophils were terminated at 1 hour and 24 hours after infection to determine the intracellular survival of H37Rv. Graph shows mean ± standard error.(a): Represents foldincrease in intracellular growth of H37Rv in neutrophils treated with NAC and L-GSH. (b): Determination of neutrophil viability following infection with H37Rv and treatment with NAC and L-GSH by quantifying DAPI stained cells ().
