Cysticerci Drive Dendritic Cells to Promote In Vitro and In Vivo Tregs Differentiation
Table 1
Levels of cytokines in supernatants from in vitro DC differentiation and in vitro regulatory T cell induction.
IFN-
TNF-
IL-10
IL-6
IL-2
TGF-
DC nonstimulated
0.39 ± 0.88
3.98 ± 3.82
3.17 ± 1.79
>5000
0.34 ± 0.77
406.50 ± 191.69
Control medium 10% HSAB
3.55 ± 0.51
5.78 ± 1.79
5.12 ± 1.50
>5000
1.59 ± 1.41
ND
differentiation in the presence of
Dexamethasone
0
3.12 ± 0.47
30.84 ± 5.82a
>5000
0
545.17 ± 289.37
Taenia crassiceps
0.78 ± 1.75
2.98 ± 1.75
3.77 ± 1.76
>5000
0.72 ± 0.98
341.50 ± 238.19
Taenia solium
0
4.58 ± 4.44
7.31 ± 4.88
>5000
0
436.00 ± 243.95
Treg induction from naïve CD4+ cells
DC nonstimulated, nonpulsed (Ag−)
0.76 ± 1.31
1.21 ± 1.07
0.99 ± 0.93
2.58 ± 0.70
0.45 ± 0.78
124.60 ± 5.35
DC nonstimulated pulsed (Ag+)
0.78 ± 1.75
2.06 ± 0.88
1.22 ± 1.12
2.78 ± 0.60
0.43 ± 0.95
93.07 ± 16.98
Dexamethasone nonpulsed (Ag−)
0.63 ± 1.26
1.68 ± 0.34
1.01 ± 1.18
2.97 ± 2.02
0.39 ± 0.78
95.49 ± 19.66
Dexamethasone pulsed (Ag+)
1.00 ± 1.74
2.49 ± 0.36
2.32 ± 1.36
2.78 ± 1.10
1.38 ± 1.27
98.63 ± 26.39
T. crassiceps nonpulsed (Ag−)
1.23 ± 2.13
2.09 ± 0.79
1.02 ± 1.76
8.15 ± 1.26
1.31 ± 1.21
99.22 ± 31.64
T. crassiceps pulsed (Ag+)
1.26 ± 1.81
2.18 ± 0.38
1.44 ± 0.81
29.34 ± 46.75
0.43 ± 0.95
119.80 ± 42.56
T. solium nonpulsed (Ag−)
4.50 ± 2.61
2.35 ± 0.68
1.48 ± 2.10
56.99 ± 5.21
12.14 ± 11.79
111.00 ± 3.54
T. solium pulsed (Ag+)
1.73 ± 2.45
2.28 ± 0.68
1.95 ± 0.09
11.68 ± 6.02
6.61 ± 6.16
78.50 ± 21.21
Control T cell
1.54 ± 1.41
1.90 ± 0.48
1.77 ± 0.99
2.01 ± 0.45
0.88 ± 1.20
101.00 ± 16.58
Significantly different () compared to nonstimulated DC. AB sera from healthy donors were used in DC differentiation experiments. Levels of cytokines in the control medium supplemented with 10% HSAB are shown.
