Inflammatory Chemokine Expression via Toll-Like Receptor 3 Signaling in Normal Human Mesangial Cells
Figure 2
Immunoreactivity of MDA5 and RIG-I in biopsy specimens (Imaizumi et al. Tohoku J Exp Med 2012). Stored kidney specimens in good condition obtained from cases of diffuse proliferative lupus nephritis, proteinuric IgA nephropathy, minimal change nephrotic syndrome, and nutcracker syndrome were used for immunofluorescent study of MDA5 and RIG-I expression. After blocking by incubation with 1% goat serum, the slides were incubated with an anti-MDA5 antibody (1 : 100) or an anti-RIG-I antibody (1 : 1000). Intense MDA5 immunoreactivity was detected in MCs of the specimens from diffuse proliferative lupus nephritis and proteinuric IgA nephropathy, while the expression in nonimmune complex-mediated renal diseases was undetectable. Interestingly, RIG-I immunoreactivity was only in diffuse proliferative lupus nephritis.
