Glioma-Associated Antigen HEATR1 Induces Functional Cytotoxic T Lymphocytes in Patients with Glioma

Figure 5

HEATR1-specific-peptide CTLs kill HLA-A∗02+ gliomas that express HEATR1. (a) RNA was isolated from three GBM cell lines and mRNA expression of HEATR1 was investigated by RT-PCR. (b) The values shown represent the mean ± SD of triplicate assays from PBMCs of patient number 323. U87, SHG66, and A172 were loaded with or without peptides and used as target cells in a LDH-release assay. The results showed that 6-peptides-stimulated PBMCs significantly lysed U87 and SHG66 target cells expressing both HEATR1 and HLA-A∗02 but not A172 cells that do not express HLA-A∗02 at an E/T ratio of 10 : 1. (c) Six-peptides-stimulated PBMCs from patient number 323 also significantly lysed the U87 and SHG66 GSCs at an E/T ratio of 10 : 1. Statistical differences between two groups were evaluated by the unpaired Student’s 𝑡-test.