Inflammation and ER Stress Downregulate BDH2 Expression and Dysregulate Intracellular Iron in Macrophages

Figure 2

ER stress markers are induced in macrophages by tunicamycin. THP-1 cells ( cells/mL) were treated with tunicamycin (10 μg/mL) or DMSO overnight prior to LPS exposure (40 ng/mL) for 6 hr. RNA was extracted and ER stress markers CHOP (a), hsXBP (b), and GRP78 (c) gene expressions were assessed by quantitative RT-PCR normalized to β-actin. The fold change in CHOP and hsXBP gene expression was calculated in reference to DMSO treated control THP-1 cells using the ΔΔCT method and presented here normalized to no treatment control. Error bars represent the SD from the mean of three independent experiments. values were calculated in reference to no treatment (DMSO only) control using one-way ANOVA followed by Bonferroni multiple comparisons post hoc analysis. values annotated ; ; ; were not significant (NS).

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