Inflammation and ER Stress Downregulate BDH2 Expression and Dysregulate Intracellular Iron in Macrophages

Figure 5

NGAL gene expression is massively upregulated by ER stress. (a) THP-1 cells ( cells/mL) were treated with tunicamycin (10 μg/mL) or DMSO overnight prior to LPS exposure (40 ng/mL) for 6 hr. (b) THP-1 cells ( cells/mL) were treated with tunicamycin doses (5, 2.5, or 0.5 μg/mL) as in panel (a) or tunicamycin followed by LPS (40 ng/mL). RNA was extracted and NGAL gene expression was assessed by quantitative RT-PCR normalized to β-actin. The fold change in NGAL gene expression was calculated in reference to DMSO treated control THP-1 cells using the ΔΔCT method and presented here normalized to vehicle (DMSO) treatment control. Error bars represent the SD from the mean of three independent experiments. values were calculated in reference to no treatment (DMSO only) control using one-way ANOVA followed by Bonferroni multiple comparisons post hoc analysis. values annotated ; ; ; were not significant (NS).

(a)

(b)