Inflammation and ER Stress Downregulate BDH2 Expression and Dysregulate Intracellular Iron in Macrophages

Figure 6

ER stress and inflammation induce heme oxygenase-1 (HO-1) gene expression in macrophages. THP-1 cells ( cells/mL) were treated with tunicamycin (10 μg/mL) or DMSO overnight prior to LPS exposure (40 ng/mL) for 6 hr. RNA was extracted and HO-1 gene expression was assessed by quantitative RT-PCR normalized to β-actin. The fold change in OH-1 gene expression was calculated in reference to DMSO treated control THP-1 cells using the ΔΔCT method and presented here normalized to no treatment control. Error bars represent the SD from the mean of three independent experiments. values were calculated in reference to vehicle treatment using one-way ANOVA followed by Bonferroni multiple comparisons post hoc analysis. values annotated ; ; were not significant (NS).