Inflammation and ER Stress Downregulate BDH2 Expression and Dysregulate Intracellular Iron in Macrophages

Table 1

Cytokine and chemokine release from THP-1 cells.

Cytokine/chemokine release (pg/mL)	No treatment	Tunicamycin (10 µg/mL)^a,b	LPS (40 ng/mL)^a,b,c,#	Tunicamycin + LPS^a,c
IL-1β	11.4 ± 0.1	411.6 ± 25.7	515 ± 54	415.3 ± 62
value < 0.0001^a		NS^b		NS^c
IL-13	13.1 ± 1.3	18 ± 2.5	19.43 ± 2.5	21.24 ± 2.2
value = 0.0142^a		NS^b		NS^c
IL-6	5.69 ± 0.1	23.6 ± 13.7	47.2 ± 20	225 ± 31
value < 0.0001^a		NS^b		<0.0001^c
IL-12p40	2.9 ± 2.1	190 ± 232	1913 ± 228	323 ± 234
value < 0.0001^a		<0.0001^b		<0.0001^c
GM-CSF	ND^!	2.02 ± 0.7	2.98 ± 0.39	3.7 ± 0.38
value < 0.0001^a		NS^b		NS^c
IFNα	7.81 ± 1.4	21.8 ± 6.2	35.9 ± 0.73	28.8 ± 4.6
value = 0.0001^a		<0.01^b		NS^c
IL-1R antagonist	11.33 ± 6.6	430.9 ± 114	1071 ± 164	528 ± 41.2
value < 0.0001^a		<0.0001^b		<0.001^c
TNFα	0.714 ± 0.15	40.2 ± 58	245.2 ± 152	30.8 ± 38
value = 0.025^a		NS^b		NS^c
IL-2R	2.27 ± 3.0	47.9 ± 35.8	122.8 ± 7.7	102.7 ± 10.4
value = 0.0002^a		<0.001^b		NS^c
RANTES	67 ± 48.7	373 ± 169	756 ± 82	480 ± 112
value = 0.0005^a		<0.01^b		NS^c
MIP1α	34.29 ± 0	1010 ± 1493	5404 ± 394	2279 ± 397
value = 0.001^a		<0.001^b		<0.01^c
MIP1β	9.8 ± 0	1913 ± 1750	8295 ± 1895	3902 ± 551
value = 0.0003^a		<0.001^b		<0.01^c
MCP-1	5.09 ± 2.2	274 ± 94	336 ± 68	417 ± 118
value = 0.0015^a		NS^b		NS^c
IP-10 (CXCL10)	4.0 ± 3.5	67.5 ± 56	104.4 ± 67	55.1 ± 29
value = 0.139^a		NS^b		NS^c
IL-8	15.4 ± 5.7	6832 ± 542	7034 ± 848	6832 ± 598
value < 0.0001^a		NS^b		NS^c

ND: not detected.
^a values calculated in reference to no treatment using one-way ANOVA.
^b values of LPS treatment (^#) compared to tunicamycin using Bonferroni’s multiple comparison test.
^c values of LPS treatment (^#) compared to tunicamycin + LPS using Bonferroni’s multiple comparison test.
NS: not significant.