Regulatory Lymphocytes Are Key Factors in MHC-Independent Resistance to EAE
Figure 5
(a) Intracellular production of interleukin-17 (IL-17) by spleen CD4+ T cells. We analyzed these cells in series of seven mice per condition. Splenocytes from C57BL/6 and CD1 mice were cultured in presence of phorbol-12-myristate-13-acetate (PMA) and ionomycin during four hours and the percentage of CD4+ T cells showing intracellular production of IL-17 was analyzed by flow cytometry. We studied nonimmunized mice (NI) and mice immunized with MOG35–55 (MOG) and with PLP139–151 (PLP). Only C57BL/6 mice that developed EAE after immunization with MOG35–55 displayed a significant increase in the percentage of CD4+ T cells producing IL-17. (b) Representative dot plots of IL-17 intracellular production by CD4+ T cells from MOG35–55-immunized CD1 and C57BL/6 mice are shown. (c) Serum concentrations of IL-17A in nonimmunized mice (NI) and mice immunized with MOG35–55 (MOG).
