Differential Transcript Profiles of MHC Class Ib(Qa-1, Qa-2, and Qa-10) and Aire Genes during the Ontogeny of Thymus and Other Tissues

Table 1

Relative Quantification of gene expression profiles of H2-Q7, H2-Q10, H2-T23 and Aire throughout the development of C57BL/6 mice. The lines are representative of the mean values ​ ​of 2^−ΔΔCt. Tissue samples were obtained in triplicate from different animals for each age analyzed. Each experiment was independently performed at least three times. Data were analyzed statistically by One-way ANOVA followed by the Bonferroni multiple comparison test. The comparison was performed between the transcript levels of one target gene in the same tissue but at different ages. Values close to the level of significance are marked with (∗). Values ​ ​near the significance level (𝑃<0.05) are marked with (†), (𝑃<0.01) with (•) and (𝑃<0.001) with (∗). was utilized.

	Thymus				Liver				Intestine
	H2-Q7 (Qa2)	H2-T23 (Qa1)	H2Q10 (Qa10)	Aire	H2-Q7 (Qa2)	H2-T23 (Qa1)	H2Q10 (Qa10)	Aire	H2-Q7 (Qa2)	H2-T23 (Qa1)	H2Q10 (Qa10)	Aire
E14.5	0.0102	0.3810	0.0344	0.1617	0.1355	0.0735	0.0187	0.0000	0.0515	0.0080	0.0365	0.1550
E15.5	0.0966	0.1100	0.1967 ^†	0.2500	0.1465	0.0725	0.0286	0.0000	0.0340	0.0045	0.0365	0.2893
E16.5	0.0920	0.2220	0.6565 *	0.9500 *	0.088	0.1447	0.0765	0.0000	0.0333	0.0203	0.1503	0.2180
E17.5	0.0785	0.3020	0.0200	0.9850 *	0.09	0.1160	0.0330	0.0000	0.0426	0.0156	0.1273	0.7205
E18.5	0.0066	0.2060	0.0172	0.1075	0.1008	0.1535	0.1156	0.0000	0.0246	0.0296	0.2140	0.3527
E19.5	0.1070	0.2300	0.3683 ^†*	0.1367	0.0625	0.1330	0.0956	0.0000	0.0185	0.0355	0.7255 ^†•*	0.3760
E20.5	0.0560	0.3540	𝟎.𝟓𝟓𝟎𝟓^∗	0.0200	0.112	0.1308	0.1775 ^†	0.0000	0.0280	0.0660	0.949 ^†*	0.3000
1	0.1645 ^†	0.4323 ^†•	𝟎.𝟗𝟗𝟓𝟓^∗	0.0733	0.0625	0.2020	0.0370	0.0000	0.0267	0.1137	0.2960	0.2175
5	0.0243	0.6658 ^†•*	𝟎.𝟓𝟐𝟓𝟎^∗	0.1700	0.2225 ^†	0.6850 ^•*	0.0917	0.0000	0.0695	0.1997	0.2400	0.7715 ^†
10	0.0166	0.3185	0.0172	0.0200	0.332 ^•*	0.6167 ^†•	0.1347	0.0000	0.1900	0.4580	0.0080	0.3810
15	0.0320	0.6668 ^•*	0.0423	0.1850	0.185	0.6743 ^†•*	0.1513	0.0000	0.1905	0.5445 ^†	0.0230	0.2750
45	𝟎.𝟐𝟏𝟗𝟎^†	0.4633 ^•	0.0563	0.0866	𝟎.𝟗𝟗𝟗𝟓^∗	𝟎.𝟔𝟑𝟖𝟎^†•	0.7717 *	0.0000	0.6305 ^•*	0.3805	0.0530	0.3080
60	𝟎.𝟗𝟏𝟓𝟖^∗	0.2993	0.0425	0.0360	𝟎.𝟗𝟏^∗	𝟎.𝟖𝟑𝟖𝟓^∗	0.5723 *	0.0000	𝟎.𝟗𝟏𝟕𝟓^∗	0.699 ^†•*	0.0360	0.1310
	Spleen				Placenta				Brain
	H2-Q7 (Qa2)	H2-T23 (Qa1)	H2Q10 (Qa10)	Aire	H2-Q7 (Qa2)	H2-T23 (Qa1)	H2Q10 (Qa10)	Aire	H2-Q7 (Qa2)	H2-T23 (Qa1)	H2Q10 (Qa10)	Aire
E14.5	—	—	—	—	0.0326	0.2435	0.7957 ^†	0.0210	0.0580	0.0350	0.0170	0.6820
E15.5	—	—	—	—	0.0450	0.6507	0.2425	0.0596	0.1823	0.0230	0.0330	0.7630
E16.5	—	—	—	—	0.0413	0.3227	0.2490	0.0693	0.1673	0.0060	0.0370	0.3703
E17.5	—	—	—	—	—	—	—	—	0.1453	0.0185	0.0170	0.2370
E18.5	—	—	—	—	—	—	—	—	0.1570	0.0140	0.0150	0.2220
E19.5	—	—	—	—	—	—	—	—	0.1375	0.0120	0.0535	0.3280
E20.5	—	—	—	—	0.0233	0.2803	0.0430	𝟎.𝟖𝟕𝟔𝟕^∗	0.3205	0.0140	0.6965	0.6355
1	0.0165	0.1047	𝟎.𝟐𝟏𝟎𝟑^∗	0.0295	—	—	—	—	0.3030	0.0000	0.0855	0.4105
5	0.0085	0.0965	0.0190	0.0836	—	—	—	—	0.0000	0.0880	0.0140	0.1940
10	0.0343	0.2217	0.0126	0.0126	—	—	—	—	0.6560	0.0510	0.0270	0.1980
15					—	—	—	—	0.0440	0.0050	0.0330	0.2750
45	0.1667	0.5817	0.0533	0.0806	—	—	—	—	0.1110	0.0250	0.0530	0.1820
60	𝟎.𝟕𝟔𝟔𝟓^∗	0.5973	0.0553	0.2073 ^•*	—	—	—	—	0.3530	0.0400	0.1165	0.1355

^†(𝑃<0.05); ^•(𝑃<0.01); ^∗(𝑃<0.0001).