Research Article

Osteodifferentiated Mesenchymal Stem Cells from Bone Marrow and Adipose Tissue Express HLA-G and Display Immunomodulatory Properties in HLA-Mismatched Settings: Implications in Bone Repair Therapy

Figure 8

Both BM- andAT-derived MSCs when committed to osteodifferentiation process keep their immunosuppressive properties. PBMC from healthy individuals were used as responder cells towards irradiated used as stimulating cells in presence of either osteodifferentiated BM-derived MSCs (Os-MSC-BM) or AT-derived MSCs (Os-MSC-AT) that were pretreated with IFNγ and TNFα (IFNγ/TNFα) or not and used as third-party cells at various responder : stimulator : MSC ratios. Results are given as mean percentage of alloproliferation inhibition ± s.e.m. when compared to PBMC using PBMC from 3 and 5 distinct healthy donors in BM- and AT-derived MSCs experiments, respectively.
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