Selective Depletion of Regulatory T Cell Subsets by Docetaxel Treatment in Patients with Nonsmall Cell Lung Cancer

Figure 1

aTreg but not rTreg and non-Treg cells increased in NSCLC patients. (a) CD4⁺Foxp3⁺ T cells and three subsets of Treg cells from PBMCs were isolated and analyzed by FACS. (b) The percentages of Treg cells (CD4⁺Foxp3⁺ T (total Treg) cells, CD4⁺CD45RA⁻Foxp3^hi (aTreg) cells, CD4⁺CD45RA⁻Foxp3^lo (non-Treg) cells, and CD4⁺CD45RA⁺Foxp3^lo (rTreg) cells) in CD4⁺ T cells were calculated after FACS analysis. HD: healthy donor, 𝑛=20; NSCLC: nonsmall cell lung cancer, 𝑛=40. Each dot represents one individual sample. ^∗∗𝑃<0.01 and ^∗∗∗𝑃<0.001 for statistical analysis by Student’s 𝑡-test.

(a)

(b)