Role of Lysosomes in Silica-Induced Inflammasome Activation and Inflammation in Absence of MARCO
Figure 8
Increased cholesterol uptake in WT AM in response to silica. Isolated AM were loaded with TopFluor cholesterol (1 μg/mL) for 24 hrs prior to silica (50 μg/mL) exposure in RPMI media with 5% delipidated FBS. Silica exposure was 4 hrs in regular RPMI culture media. The wells were read in a microplate reader at 488 nm ex/525 nm em. Asterisk indicates statistical significance at compared to baseline WT response, .
