Current Concepts and Future Directions for the Assessment of Autoantibodies to Cellular Antigens Referred to as Anti-Nuclear Antibodies
Table 3
Commercially available immunoassays for ANA testing.
Antigen
Automated screening assays
Multiplex assays
Line immunoassays/dot blots
EliA CTD
EliA Symphony
QUANTA Flash ENA7
QUANTA Flash CTD Screen Plus
Alegria ANA Detect
Bio-Plex 2200 ANA ALBIA
AtheNA Multi-Lyte Anti-Nuclear Antibodies (ANA)
FIDIS Connective 10
ANA Profile
ImmcoStripe ANA Advanced
ANA12 IgG BlueDot
ANA-12 Pro (BLOT)
recomLine ANA/ENA IgG
ANA Lia MAX
Thermo Scientific
Thermo Scientific
INOVA Diagnostics
INOVA Diagnostics
Orgentec
Bio-Rad
Zeus
Theradiag
Euroimmun
IMMCO Diagnostics
D-Tek
AESKU Diagnostics
MIKROGEN Diagnostics
HUMAN Diagnostics
U1-RNP
X
X
X
X
X
X
X
X
X
X
U1-RNP/Sm
X
X
X
X
X
X
U1-RNP 68 kDa
X
X
X
U1-RNP A
X
X
X
U1-RNP C
X
X
X
SS-A/Ro60
X
X
X
X
X
X(i)
X
X
X
X
X
X
X
X
Ro52/TRIM21
X
X
X
X
X
X(i)
X
X
X
X
X
SS-B/La
X
X
X
X
X
X
X
X
X
X
X
X
X
X
Centromere
X
X
X#
X
X
X
X
X
X#
X
X
X
Scl-70/topo I
X
X
X
X
X
X
X
X
X
X
X
X
X
Jo-1
X
X
X
X
X
X
X
X
X
X
X
X
X
X
Fibrillarin/U3RNP
X
RNA-Pol III
X
X
Ribosomal P
X
X
X
X
X
X
X
X
X
X
PM/Scl
X
X
X
X
PM/Scl-75
X
PM/Scl-100
X
X
PCNA
X
X
X
X
X
X
X
Mi-2
X
X
X
X
X
Sm
X
X
X
X
X
X
X
X
X
X
X
dsDNA
X
X
X
X
X
X
X
X
X
X
Nucleosome
X
X
X
X
X
Histone
X
X
X
X
X
X
X
Ku
X
X
X
X
X
SRP54
X
AMA-M2
X
X
X
Note: some companies offer several line immunoassays for ANA detection. Most comprehensive assays from different companies are shown. AMA: Anti-mitochondrial antibodies; PCNA: Proliferating cell nuclear antigen; SRP: signal recognition particle. NOTE: RNP and RNP/Sm contain the subunits RNP-A, RNP-C and RNP-68 kDa.
(i)Reported outside the United States as Ro60 and Ro52 and as SS-A in the United States.
#Contains CENP-A and CENP-B.
