IFN-γ-Secreting-Mesenchymal Stem Cells Exert an Antitumor Effect In Vivo via the TRAIL Pathway
Figure 2
Construction of IFN-γ-modified MSCs. (a) The lentiviral vector pLV/Final-puro-EF1α- IFN-γ was identified by PCR. Lane 6/7: negative controls; lanes 2–5: amplification fragments of the IFN-γ coding sequence; lanes 8–11: amplification fragments of the EF1α promoter sequence. (b) The morphology of MSCs hrGFP and MSCs IFN-γ. Scale bar: 200 μm. (c) The expression of IFN-γ was assayed by ELISA. (d) TRAIL expression in MSCs IFN-γ was determined by Western blotting analysis. Lane 1: negative control; lane 2/3: MSCs IFN-γ at P2 and P5 passages.
