Humoral Autoimmune Responses to Insulin-Like Growth Factor II mRNA-Binding Proteins IMP1 and p62/IMP2 in Ovarian Cancer

Figure 3

Representative immunofluorescence staining pattern of anti-IMP1 and anti-p62/IMP2 positive ovarian cancer sera. (a) A normal human serum (NHS) was used as negative control; (b) monoclonal anti-IMP1 or anti-p62/IMP2 antibody that demonstrated a cytoplasmic immunofluorescence staining pattern was used as positive control; (c) representative anti-IMP1 or anti-p62/IMP2 positive ovarian cancer sera demonstrated an intense cytoplasmic immunofluorescence staining pattern; (d) the same ovarian cancer serum that was used in panel (c) was postabsorbed with recombinant IMP1 or p62/IMP2 protein. The fluorescent cytoplasmic signal was remarkably decreased.