Multiple Roles of Myd88 in the Immune Response to the Plague F1-V Vaccine and in Protection against an Aerosol Challenge of Yersinia pestis CO92 in Mice
Figure 7
Antibody titers against the F1-V vaccine in (a) C3H/HeN wild-type and Tlr4 mutant C3H/HeJ mice, and (b) C57BL/6 wild-type and Myd88-deficient C57BL/6 mice vaccinated with F1-V before Y. pestis CO92 aerosol challenge. (a) There were 4 groups of mice with 10 in each group: (1) Wt+adjuvant, (2) Tlr4 mutant+adjuvant, (3) Wt+F1-V (2.9 μg), and (4) Tlr4 mutant+F1-V (2.9 μg). Antibody titers are reported from serum collected 22 days after a boost vaccination. (b) There were 3 groups of mice with 15 in groups 1 and 2 and 14 in group 3: (1) Wt+adjuvant, (2) Wt+F1-V (2.5 μg), (3) Myd88-deficient mice+F1-V (2.5 μg). Antibody titers are reported as geometric mean with geometric standard error of the mean. Significant differences between the antibody class or subclass are reported above the respective bar between the (a) Wt+F1-V and Tlr4 mutant+F1-V or (b) Wt+F1-V and Myd88-deficient+F1-V.
