Autoantibodies Targeting AT1 Receptor from Patients with Acute Coronary Syndrome Upregulate Proinflammatory Cytokines Expression in Endothelial Cells Involving NF-κB Pathway

Figure 4

IL-6, VCAM-1, and MCP-1 protein expression in human endothelial cells stimulated by AT1-AAs. After incubation with AT1-AAs for 0, 1, 4, 8, 12, and 24 hours or human nonspecific IgG from the patients for 12 hours as a negative control, the IL-6, VCAM-1 and MCP-1 (panel (a)) protein levels were determined by Western blot analysis. -axis represents the relative protein levels, -actin used as a protein loading control. Panel (b) was the summarized data from 5 independent experiments; versus 0 hour (control); . (nonspecific IgG group ).

(a)

(b)