Protection against Experimental Melioidosis following Immunisation with a Lipopolysaccharide-Protein Conjugate
Figure 4
The bacterial burdens of spleens 48 hours after infection. Mice were vaccinated three times at two-week intervals with 10 μg of either the TetHc-LPS conjugate (∙), LPS alone (■), a mix of unconjugated LPS and TetHc (▲), TetHc only (◆), or PBS (▾) and challenged five weeks after the final vaccination with approximately 40 MLD of B. pseudomallei K96243. Forty-eight hours after challenge, mice were culled and spleens were removed and assayed for bacterial burden. Data is shown for replicate studies and was analysed by univariate linear model. Significant differences are shown. Where no markers are present, was greater than 0.05.
