A Mycobacterium bovis BCG-Naked DNA Prime-Boost Vaccination Strategy Induced CD4⁺ and CD8⁺ T-Cell Response against Mycobacterium tuberculosis Immunogens

Figure 3

Analysis of the percentages of CD4⁺ and CD8⁺ cells (%). Animals were immunized and killed; the splenocytes were collected and handled as described in Section 2. Flow cytometry was used to determine the proportions of splenocyte subsets, and the results are presented as mean ± standard errors. ^∗𝑃<0.01 versus PBST, BCG-C, BCG-P, BCG-C +pcDNA3.1, or BCG-P+pcDNA3.1 groups. ^#𝑃<0.05 versus BCG-P+3.1-69 or BCG-P+ 3.1-72 group. ^◊𝑃<0.05 versus BCG-P+3.1-69, BCG-C+3.1-72, or BCG-P+3.1-72 groups.