A Mycobacterium bovis BCG-Naked DNA Prime-Boost Vaccination Strategy Induced CD4⁺ and CD8⁺ T-Cell Response against Mycobacterium tuberculosis Immunogens

Figure 4

Analysis of IFN-γ released from splenocytes. After animals were harvested; splenocytes were collected and prepared as described in Section 2. IFN-γ concentration in the medium was measured by ELISA kit according to the manufacturer’s protocol. Triplicate tests were done for each medium sample. ^∗𝑃<0.01 versus PBST, BCG-C, BCG-P, BCG-C+pcDNA3.1, or BCG-P+pcDNA3.1 groups. ^#𝑃<0.05 versus BCG-P+3.1-69 or BCG-P+3.1-72 group. ^◊𝑃<0.05 versus BCG-C+3.1-69 group. ^☆𝑃<0.05 versus BCG-C+3.1-69 or BCG-P+3.1-69 group.