Sonic Hedgehog Signaling Drives Proliferation of Synoviocytes in Rheumatoid Arthritis: A Possible Novel Therapeutic Target
Figure 5
Cyclopamine decreased expression of Shh, Ptch1, Smo, and Gli1 mRNA in cultured FLS from RA. Cells were stimulated, treated, and harvested as described in Section 2. cDNA was synthesized and used for quantitative PCR. Relative quantification of gene expression was performed by the method. Means ± S.D. of 3 independent experiments are shown. versus control. Control group: FLS from RA patients were cultured in DMEM supplemented with 10% FBS. Differences between means of two groups were performed by independent-samples -test.
