Sonic Hedgehog Signaling Drives Proliferation of Synoviocytes in Rheumatoid Arthritis: A Possible Novel Therapeutic Target
Figure 7
Cyclopamine decreased the viability of cultured FLS from RA patients. Cells were stimulated, treated, and harvested as described in Section 2. (a) Cell proliferation was determined using the Cell Counting Kit-8. The cell proliferation rate in cyclopamine-treated FLS was significantly lower than that in the control group ()% () ((b), (c)). The proportion of cells in the G1 phase was significantly higher in cyclopamine-treated FLS than in controls, indicating cell cycle arrest (). (d) In cyclopamine-treated FLS the percentage of cells in the phase was significantly reduced, indicating inhibition of cell proliferation (). Results in (a), (c), and (d) show the means ± S.D. of three independent experiments.
