Research Article
Expression of Shelterin Component POT1 Is Associated with Decreased Telomere Length and Immunity Condition in Humans with Severe Aplastic Anemia
Table 1
Target gene primer sequences and annealing temperature.
| | Gene | Primer () | Annealing temperature (°C) |
| | -Actin | F CCTCATGCCATCCTGCGTCTG
R TTGCTCGAAGTCTAGGGCAACATAG | 58 | | TRF1 | F AAGTCCTGAAAGCCCTGAATC
R TTCCTGTGCCTCCAAAATCT | 58 | | TRF2 | F CTGTATTCATTTTGCTAACTTAG
R ATTCTTAACACTCTCTAGAGTTG | 55 | | TIN2 | F CTTCATTCCTACTAAACTACTTG
R ACTGTAGAGACAGTTCTAGACCT | 57 | | POT1 | F CAGAAAAGTGTGGATATG
R AAGTAAAAGAAGTGTGGG | 55 | | TPP1 | F GCGTGACGTCTCACATCG
R ATCGTGATGGTTCTGCCTTC | 55 | | RAP1 | F TGTGGTGTGTGTGTGTGTGG
R CCCCACACACACCACACAC | 56 |
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