Multiplex Evaluation of Influenza Neutralizing Antibodies with Potential Applicability to In-Field Serological Studies

Figure 2

The influence of freeze-thaw cycles on pseudotype virus titre. The stability of H5N1 lacZ pseudotypes was evaluated by subjecting aliquots of virus to 5 cycles of freeze-thaw. Results for pseudotypes bearing the rabies virus (CVS-11) and HIV-1 envelope proteins are shown for comparison. One biological replicate of each pseudotype virus was used to generate three technical replicates. Error bars represent standard deviation.