Parainfluenza Virus Type 1 Induces Epithelial IL-8 Production via p38-MAPK Signalling

Figure 1

Lytic plaque assay. The number of viral particles was determined by dilution. In a 24-well plate with HEp-2 and A549 cell cultures, each lytic plaque that develops is initially related to a single infectious virion particle. This method was performed to determine the viability of both viruses, live and inactive. (a) refers to the cell type and the characteristic of the virus (inactive and live); (b) corresponds to dilutions ranging from 10⁻¹ to 10⁻⁵; and (c) refers to plaque-forming units. This assay was performed in triplicate.