Hepatitis E rORF2p Stimulated and Unstimulated Peripheral Expression Profiling in Patients with Self-Limiting Hepatitis E Infection
Figure 4
Gene expression profiles in hepatitis E infection by TLDA. (a) Gene expression profiles using customized TLDA panel by two-way hierarchical clustering on unstimulated PBMCs from AVH-E patients, recovered individuals after normalization with healthy controls. Each colored cell in the two hit maps represents the relative levels of expression of particular genes in a study subject. Green indicates low levels and red indicates high levels of gene expression. Values for 26 selected genes were hierarchically clustered on log transformation. The corresponding gene of each cluster is listed by a gene symbol on the top side of the images. Genes were ordered according to their cluster determined by the k-means algorithm. (a) AVH-E patients denoted as A1–A33; (b) recovered individuals denoted as R1–R20 on the right side of the images. Values increase from green to red, via black. (c-d) HEV rORF2p stimulated gene expression of in vitro cultured PBMCs of AVH-E patients. The values shown are the mean fold change of rORF2p stimulated PBMCs compared with the unstimulated PBMCs. Values are expressed as mean ± standard deviation.
