A Circulating Subpopulation of Monocytic Myeloid-Derived Suppressor Cells as an Independent Prognostic/Predictive Factor in Untreated Non-Small Lung Cancer Patients

Figure 3

Representative histograms of flow cytometry analysis of (a) iNOS and (d) intracellular oxidative stress by the DCF method. Percentages of (b) iNOS and (e) ROS producing cells from healthy and NSCLC patients. Intracellular levels of (c) iNOS and (f) ROS in all tested subpopulations. The data are the mean fluorescence intensity (MFI). Intracellular ROS levels in subpopulations of MDSCs before and after PMA stimulation. Green bars, healthy controls; red bars, NSCLC patients. The gates for each dot plot and histogram are presented on the top of each box. The positive expression of markers is compared to cells without Ab staining. Colours in histograms represent the different subpopulations; pink colour, CD11b⁺CD14⁺CD15⁺CD33⁺HLA-DR⁻Lin⁻ population; black colour, CD11b⁺CD14⁺CD15⁻CD33⁺HLA-DR⁻Lin⁻, and bright blue, CD11b⁺CD14⁻CD15⁺CD33⁺HLA-DR⁻Lin⁻. Percentages indicated in the plots represent the percentages of phenotypic marker expression in the parental population, which are presented inside the brackets. The data are represented as the mean ± SEM and the values are determined by Mann-Whitney test.

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