Control of Intracellular Francisella tularensis by Different Cell Types and the Role of Nitric Oxide

Figure 3

((a) and (b)) Intracellular counts 24 hours after stimulation and infection of J774A.1 cells (a) and MH-S cells (b). Stimulant concentrations added were IFN-γ (1 μg/mL), LPS (5 μg/mL), IFN-𝛾 + LPS (1 μg/mL + 5 μg/mL), and CpG (10 μg/mL). Nitric oxide production was inhibited by 4 mM N^G-monomethyl-L-arginine added before stimulation. Values are the means and SEM from at least three independent experiments, with the starting infection given a value of 1% and the maximal growth achieved (in unstimulated cells) as 100%. Significant differences in intracellular counts between stimulated and unstimulated cells are shown (significance *𝑃<0.05, **𝑃<0.01, and ***𝑃<0.001 by Student’s 𝑡-test).

(a)

(b)