Roles of TLR3 and RIG-I in Mediating the Inflammatory Response in Mouse Microglia following Japanese Encephalitis Virus Infection

Figure 10

Viral load in TLR3 and RIG-I knockdown cells. BV-2 cells were transfected with RIG-I shRNA (RIG-I KD), TLR3 shRNA (TLR3 KD), or negative control shRNA (CTR) and then infected with JEV at an MOI of 0.01. Cells were collected at different time points, and total RNA was isolated. The number of copies of the JEV C gene was determined with qRT-PCR. Error bars represent the mean ± standard error from three independent experiments.