Roles of TLR3 and RIG-I in Mediating the Inflammatory Response in Mouse Microglia following Japanese Encephalitis Virus Infection

Figure 3

Nuclear localization of AP-1 and NF-κB in JEV-infected BV-2 cells. BV-2 cells were either mock infected or infected with JEV at an MOI of 1. Poly(I : C) was added as a positive control. (a) AP-1 and NF-κB in the nucleus and whole-cell extracts were detected with western blotting at 5 hpi. (b, c) These proteins were quantified with immunoblot scanning and normalized to the amount of lamin A or β-tubulin. The ratios of protein levels in the nucleus to levels in whole-cell extracts were calculated. Error bars represent the standard deviation of results from three independent assays (^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001).

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