Roles of TLR3 and RIG-I in Mediating the Inflammatory Response in Mouse Microglia following Japanese Encephalitis Virus Infection

Figure 5

Knockdown of RIG-I attenuates the activation of p38MAPK and ERK in JEV-infected BV-2 cells. BV-2 cells were transfected with RIG-I shRNA (RIG-I KD) or negative control shRNA (CTR) and then infected with JEV at an MOI of 1. (a, c) Phospho-ERK (pERK) and phospho-p38MAPK (pp38MAPK) were detected with western blotting at 5 hpi. (b, d) The protein levels were quantified with immunoblot scanning and normalized to the amount of β-tubulin. Error bars represent the standard deviation of results from three independent assays (^∗𝑃<0.05; ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001).

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